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BLAST means Basic Local Alignment Search Tool. It is a sequence analytical and comparison tool hosted by the National Center for Biotechnology Information (NCBI)
It is used to do a variety of sequence comparison analysis. It can be used for comparing both the protein and nucleotide sequence obtained from the same or different sources
Doing simple sequence comparison search with the NCBI BLAST tool
Before conducting any search with the BLAST tool, its important that you understand specifically the type of information you intend to obtain. Is it homologs, paralogs or just a comparison of a segment of the amino acid or nucleotide sequence? Homology search is a search that is conducted by comparing your protein (for instance) with the database of all the protein known to man.
Paralog search is when you want to search within the database of a specific organism. For instance, if you want to obtain the set of paralogs of a protein X obtained from rat, it means that you will configure your search to scan within the database of rats only.
FIG 1
FIG 2
FIG 3
Consult the site documentation and help for more information on using the BLAST Tool.
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