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Author Topic: DILUTION TECHNIQUES  (Read 6019 times)
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Francis Umeoguaju
Expert in Bioscience Issues
Posts: 657

« on: April 10, 2010, 07:47:37 am »

Dilution technique

There are many terms used to describe dilution. Such as 10 times dilution, one in ten dilutions, 1:9 dilution etc, all these term are referring to the same thing which involves taking one part of the sample and addition it to 9 parts of distilled water. In this case the dilution factor is 10.

Preparing a diluted sample involves taking some part of the sample and adding it to a specified amount of water or other diluents.

Essentially to prepare a N time dilution of a solution from a given volume (Xml) of the sample, the total resultant volume of the diluted solution will be given as N x Xml. It implies that water is added to Xml of the sample until it gets to (N x X)ml.  In this case we have prepared a, N time dilution of the sample or a one in N dilutions or a X: (N-1) dilution.

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