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Author Topic: MICROBIAL CULTURE MEDIA- Preparatory Techniques  (Read 5158 times)
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Francis Umeoguaju
Expert in Bioscience Issues
Posts: 657

« on: April 10, 2010, 08:13:00 am »

A culture media is any food media that has all it takes to support the growth of microorganism. They usually come in different commercial type. The type that I am familiar with includes; the sabouraud dextrose Agar, soybeans extract Agar Etc. Choice of culture media for a particular work depends on the organism that would be grown on it. Different organism has difference preference for culture media. The media constituent needs to be as close as possible to the substrates found in the organisms natural environment. For instance, microorganism found in the blood needs to be cultured with media that contains blood meal extract.
Preparation of commercial sabouraud Dextrose Agar (SDA)

Depending on the volume of the media required, some amount of the SDA is weighed and dissolved in water so that the SDA concentration is 6.2% (w/v). When the mixtures are thoroughly mixed, they are placed in a cotton wool-covered conical flask and are then antoclaved.

The sterilized SDA solution is poured in Petri dished and maketney bottles while it’s still hot since it gels as it cools.
Preparation of  soybeans extract Agar

Some amount of soybeans is weighed and soaked in water  for 24hrs, the essence is to activated the inherent proteases in the seed. The harvested seeds are then blended in thrice their weight of water. The homogenate  is then filtered with  Muslims  cloth. The filtrate is incubated with a proteolytic enzyme for 24hrs at 450C. After the incubation, the  precipitated material is made 2% and 2.5% with Noble agar and lactose respectively.

When a homogenous mixture is obtained, the solution is then autoclaved and poured into plates and  marketney bottle while still hot.

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