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Francis Umeoguaju
Expert in Bioscience Issues
Posts: 657

« on: April 10, 2010, 08:17:03 am »

The aim of culturing organism is to isolate, identify & characterize a particular strain of organism from a mixed colony. Usually, the  inoculating loop is  flamed  until red hot to keep  it sterile. The inoculation loops are them used to introduce a single colony of the mixed colony into freshly prepared culture media.
The loops is used to make  streaks of the organism on the plate. The streaking is made by moving the loop in a zigzag manner over the plate. The aim of streaking is to separate the colony into single cells.
The plates are then incubated at an appropriate  temperature (usually room temperature for most organism  encountered). After a day or two, colonies are usually large enough  to be  visible  to the unaided eye.
The size, shape, texture and colour or colony, provide clues to the identification of the bacteria as do nutrient and physical  condition require for growth.

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