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Author Topic: PROTEIN ADSORPTION- Preparation of Hydroxyl Apartite gel  (Read 3534 times)
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Francis Umeoguaju
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« on: April 10, 2010, 08:35:06 am »


Hydroxyl apartite gel is obtained from the reaction of Di-pottasium hydrogen phosphate and calcium chroride. This is usually  prepared by mixing 27.36% (w/v )of Di-pottasium hydrogen phosphate (K2HPO4.3H2O) obtained by
dissolving 68.4 grams of it in 250ml of water) with 11.76% of calcium chloride dehydrate (CaCl2.2H2O) (obtained by dissolving 20.4 grams of it in 250ml  of distilled water). The resultant precipitate is mixed properly. The precipitate is then separated from the excels solution by filtering with a filter paper.

After the initial separation, the precipitate is then washed with 500ml of distilled water to wash off un-dissolved soluble precipitate. The hydroxyl Apatite is then stored for used.
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