COURSE CONTENTS
Polymerase Chain Reaction (PCR)- techniques and its application in Molecular Biology
- Amplification of DNA
- Quantitative Real Time (qRT - PCR) and its application
Recombinant DNA technology and Cloning Techniques
- Isolation and DNA purification
- Precaution for handling DNA
- DNA analysis
- DNA cloning
Molecular immunology techniques including (emphasis on research and diagnostic applications)
- ELISA methods and application
- Polyacrilamide Gel electrophoresis (PAGE),Western Blot techniques and applications
- Antibody binding assays
- Antibody neutralization assays
- Immunoflourescence
- Basic concepts in Flow cytometry and FACS analysis
Reporter - gene based diagnostic techniques
Pyrogen tests (including LAL)
Mammalian Cell Culture Techniques
- Setting up a cell culture laboratory
- Precaution for handling cells
- Manipulation and cell analysis tips
- Safety issues in Cell culture laboratory
- Primary cell culture and cell lines
- Basic operations in cell culture laboratory (subculturing, expansion, passaging,cyropreservation, and thawing)
- In vitro screening and tests on cell culture
Bioinformatics:
- Visualization tools for biomolecules
- Loading and manipulating biomolecules
- Deriving new knowledge through visualization
- Sequence alignment tools
- Alignment parameters
- Deriving new knowledge through alignment
TARGET ATTENDEES
- Postgraduate students
- Research fellows
- Lecturers in biomedical & medical sciences
- Medical laboratory scientists in research & molecular diagnostic facilities
- Research staff & fellows of Biomedicalresearch institutes & laboratories
- Research and development staff ofpharmaceutical & diagnostic firms
- Haematologist, Immunologist,Pharmacologist, Biochemists, & Microbiologists who are in research & teaching
- All students and fellows who aspire for a career in Molecular Biology,Biotechnology & Immunology
VENUE
Faculty of Pharmaceutical Sciences,
Nnamdi Azikiwe University, AguluCampus,
Anambra State, Nigeria
DATE
September 5 - 9, 2011
REGISTRATION FEE:
Twenty five thousand Naira (#25,000) per
participant
BANK DETAILS
Bank: Bank PHB Plc
Account name: National Molecular
Pathology Centre
Account Number: 1100010271
Once payment is made, please scan the teller and email it to: ic.uzochukwu@unizik.edu.ng to complete your registration.
Author