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Author Topic: protein estimation  (Read 3538 times)
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drsdnb
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« on: July 01, 2012, 07:18:23 am »


Dear sir/ madam

I have a problem with protein estimation by Lowry method. It becomes turbid sometimes, why?

Dr Shantanu Das, Ph. D.
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Francis Umeoguaju
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« Reply #1 on: July 05, 2012, 06:29:05 am »


Hello, make sure all the reagent solutions you are using are clear. Also make sure you use a clear sample to run the assay. If the sample is still clouded after running the assay, try diluting it down before running. If the problem still persist when preparing the std curve, check your reagents and make sure they are of the good grades.
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