The online community and resource center for all in life science related disciplines (BIOSCIENTISTS)
January 16, 2021, 07:36:01 pm *
Welcome, Guest. Please login or register.

Login with username, password and session length
News: Over 3000 Nigerian Life scientists are on ground & willing to provide answers to your bioscience & research questions/problems; You only need to post it online Now! (Requires very brief registration)

To be kept up to date on interesting posts in this forum,  LIKE our facebook page!
   Home   Help Login Register  
Pages: [1]
Author Topic: protein estimation  (Read 7425 times)
0 Members and 1 Guest are viewing this topic.
Newest Bioscientist
Posts: 1

« on: July 01, 2012, 07:18:23 am »

Dear sir/ madam

I have a problem with protein estimation by Lowry method. It becomes turbid sometimes, why?

Dr Shantanu Das, Ph. D.
Francis Umeoguaju
Expert in Bioscience Issues
Posts: 657

« Reply #1 on: July 05, 2012, 06:29:05 am »

Hello, make sure all the reagent solutions you are using are clear. Also make sure you use a clear sample to run the assay. If the sample is still clouded after running the assay, try diluting it down before running. If the problem still persist when preparing the std curve, check your reagents and make sure they are of the good grades.

Chances favours the trained minds
Pages: [1]
Jump to:  

Powered by MySQL Powered by PHP Powered by SMF 1.1.11 | SMF © 2006-2009, Simple Machines LLC
SMFAds for Free Forums
Valid XHTML 1.0! Valid CSS!